e7400s nebnext multiplex oligos for illumina dual index primers (New England Biolabs)

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New England Biolabs e7400s nebnext multiplex oligos for illumina dual index primers
E7400s Nebnext Multiplex Oligos For Illumina Dual Index Primers, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 572 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nebnext+dual+index+primers/NEBNext+rRNA+Depletion+Kit+v2+(Human%2FMo/pm42034059-750-201-202
Average 99 stars, based on 572 article reviews

e7400s nebnext multiplex oligos for illumina dual index primers - by Bioz Stars, 2026-10

99/100 stars

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Polymerase Chain Reaction:

Article Title: Adeno-associated viral vectors for functional intravenous gene transfer throughout the non-human primate brain.
Article Snippet: After Zymo DNA purification, we diluted the samples at 1:100 and further amplified around the variable region with ten cycles of PCR, appending binding regions for the next PCR reaction. .. Finally, we appended Illumina flow cell adaptors and unique indices using NEBNext Dual Index Primers (New England Biolabs, E7600) via ten more cycles of PCR. .. We then gel purified the final PCR products using a 2% low-melting-point agarose gel (Thermo Fisher Scientific, 16520050) and recovered the 210 bp band.

Article Title: Autoregulation of YB-1 Synthesis in Cells
Article Snippet: PCR products were purified with SPRIselect beads (NEB, USA) according to the manufacturer’s recommendations. .. The second round of PCR was performed using the primers from the NEBNext Dual Index Primers Set 1 for Illumina (NEB, USA). .. PCR products were purified with SPRIselect beads and sequenced on the NovaSeq6000 platform (Illumina) at the Skoltech Research Facilities Center (Genomics Facilities), Skolkovo Institute of Science and Technology, Russia.

Article Title: Promoter- and Enhancer-Dependent Cohesin Loading Initiates Chromosome Looping to Fold Tcrb Loci for Long-Range Recombination
Article Snippet: The adapter-ligated DNA was size-selected for 300–400 bp with AMPure XP beads and biotinylated DNA fragments were pulled down using MyOne Streptavidin T1 beads (Life Technologies). .. The final HiC library was generated with 5 PCR cycles using the NEBNext Ultra II DNA Library Prep Kit and NEBNext Dual Index primers (NEB) for Illumina sequencing. .. For comparison of WT and Eβ KO/KO , we combined two replicates of conventional HiC with two replicates of Arima HiC using the Arima-HiC Kit (A510008) according to manufacture protocol with the KAPA Hyper Prep Kit.

Article Title: Locus folding mechanisms determine modes of antigen receptor gene assembly.
Article Snippet: The adapter-ligated DNA was sizeselected for 300–400bp with AMPure XP beads and biotinylated DNA fragments were pulled down using MyOne Streptavidin T1 beads (Life Technologies). .. The final HiC library was generated with 5 PCR cycles using the NEBNext Ultra II DNA Library Prep Kit and NEBNext Dual Index primers (NEB) for Illumina sequencing. .. For comparison of our mutants, we performed two replicates each with RAG-deficient thymocytes of the WT, V1CScr/Scr, or V1PKO/KO background using the Arima-HiC Kit (A510008) according to themanufacture’s protocol with the KAPA Hyper Prep Kit.

Article Title: Adeno-associated viral vectors for functional intravenous gene transfer throughout the non-human primate brain
Article Snippet: After Zymo DNA purification, we diluted the samples at 1:100 and further amplified around the variable region with ten cycles of PCR, appending binding regions for the next PCR reaction. .. Finally, we appended Illumina flow cell adaptors and unique indices using NEBNext Dual Index Primers (New England Biolabs, E7600) via ten more cycles of PCR. .. We then gel purified the final PCR products using a 2% low-melting-point agarose gel (Thermo Fisher Scientific, 16520050) and recovered the 210 bp band.

Generated:

Sequencing:

Article Title: Adenine DNA base editor variants with reduced off-target RNA editing
Article Snippet: .. The amplicons are molecularly indexed with NEBNext Dual Index Primers (NEB) or index primers with the same or similar sequence ordered from IDT. .. Samples are combined into libraries and sequenced on the Illumina MiSeq machine using the MiSeq Reagent Kit v2 or Micro Kit v2 (Illumina).

Hi-C:

Article Title: DCAF15 control of cohesin dynamics sustains acute myeloid leukemia
Article Snippet: Hi-C was performed on 1 × 10 6 cells per replicate using the Arima High Coverage Hi-C Kit (Arima Genomics, A101030), following all manufacturer instructions. .. Hi-C libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (New England BioLabs, E7645S) and NEBNext Dual Index Primers (New England BioLabs, E7600S), with modifications to the protocol as specified by the Arima High Coverage Hi-C Kit. .. Libraries were sequenced on an Illumina NextSeq 2000 instrument using 55 × 2 paired-end cycle settings.

Article Title: DCAF15 control of cohesin dynamics sustains acute myeloid leukemia.
Article Snippet: Hi-C was performed on 1 × 106 cells per replicate using the Arima High Coverage Hi-C Kit (Arima Genomics, A101030), following all manufacturer instructions. .. Hi-C libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (New England BioLabs, E7645S) and NEBNext Dual Index Primers (New England BioLabs, E7600S), with modifications to the protocol as specified by the ArimaHigh Coverage Hi-C Kit. .. Libraries were sequenced on an Illumina NextSeq 2000 instrument using 55 × 2 paired-end cycle settings.

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